De novo establishment of an in vitro cell line culture derived from the intestine of sawflies (Hymenoptera: Diprionidae)
DOI:
https://doi.org/10.29298/rmcf.v13i69.1088Keywords:
Biological control, cellular culture, epithelial cells, Nuclear Polyhedrosis Virus, in vitro culture, intestinal epithelialAbstract
Pines, cedars and oaks forests are affected by different arthropod pests, one of which are the wasps of the family Diprionidae commonly known as sawflies. Swarms of these organisms cause defoliation on large extensions of pines and oaks along the national territory, and there is an urgent need to implement measures for the control of sawflies. One successful measure applied in other countries with similar forest pest control issues is the use of specific baculoviruses for the control of Diprionidae wasps, which has proved to be effective in the biological control of sawflies by infecting the larval stage of theses wasps, and thus increasing their mortality. However, there are no current projects in México that evaluate the identification and isolation of gamma-baculovirus destined to the biological control of forest pests. Our study addresses the technical difficulties of obtaining an intestinal cell line from Diprionidae sawflies, and we report the isolation and in vitro culture of intestinal cells.
Downloads
References
Beas-Catena, A., A. Sánchez-Mirón, F. García-Camacho, A. Contreras-Gómez and E. Molina-Grima. 2014. Baculovirus biopesticides: An overview. Journal of Animal and Plant Sciences 24(2): 362–373. http://www.thejaps.org.pk/docs/v-24-2/02.pdf (6 de junio 2021).
Beltrán V., N. E. y C. H. Gonzáles de R. 2016. Técnicas de cultivos celulares e ingeniería de tejidos. Universidad Autónoma Metropolitana, Unidad Cuajimalpa Ciudad de México, Mexico. pp. 74-89.
Cossio-Bayugar, R. and E. Miranda-Miranda. 2007. Heterologus gene expression in a cattle tick Rhipicephalus microplus embryonic cell culture. Journal of Animal and Veterinary Advances 6: 1214–1218. https://medwelljournals.com/abstract/?doi=javaa.2007.1214.1218 (6 de junio de 2021).
Cossio-Bayugar, R., C. Rojas Martinez, E. Miranda-Miranda, J. A. Alvarez- Martinez, J. V. Figueroa Millan, y C. A. Vega y Murguia. 2011. Cultivo in vitro Células Animales y sus Aplicaciones. Instituto Nacional de Investigaciones Forestales Agrícola y Pecuarias, Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Jiutepec, Mor., México. Libro técnico 3. pp. 82-95.
Garcia-Robles, I., J. Sánchez, A. Gruppe, A. C. Martínez-Ramírez, C. Rausell, M. D. Real and A. Bravo. 2001. Mode of action of Bacillus thuringiensis PS86Q3 strain in hymenopteran forest pests. Insect Biochemistry and Molecular Biology 31: 849–856. Doi: 10.1016/s0965-1748(01)00030-3. DOI: https://doi.org/10.1016/S0965-1748(01)00030-3
González G., E. y G. Sánchez M. 2018. Identificación y manejo de moscas sierrade la familia Diprionidae presentesen el centro norte de México. Comisión Nacional Forestal. Folleto técnico, pp 1-98 https://bit.ly/3FH7iS1 (6 de junio de 2021).
González G., E., F. Bonilla T., S. Q. Quiñones B., G. S. Sánchez M., F. Tafoya R., M. P. España L., J. Lozano G. y S. Robles U. 2014. Guía para la identificación de moscas sierra de la familia diprionidae presentes en el centro norte de México. Centro de Investigación Regional Norte Centro, Campo Experimental Pabellón, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarios., Aguascalientes, Ags., Mexico. Publicación especial Núm. 41. pp. 1-33.
Grzywacz, D. and S. Moore. S. (2017). Production, Formulation, and Bioassay of Baculoviruses for Pest Control. In: Lacey, L. A. (ed.). Microbial Control of Insect and Mite Pests. Elsevier. San Diego, CA, USA. pp. 109–124. DOI: https://doi.org/10.1016/B978-0-12-803527-6.00007-X
Grzywacz, D., D. Moore and J. R. Rabindra. 2014. Mass production of entomopathogens in less industrialized countries. In: Morales-Ramos, J. A., G. M. Rojas and I. D. Shapiro-Ilan (eds.). Mass production of beneficial organisms. Londo, UK. Academic Press. pp. 519–553. DOI: https://doi.org/10.1016/B978-0-12-391453-8.00015-7
King, A. M. Q., M. J. Adams, E. B. Carstens and E. J. Lefkowitz (eds.). 2012. Family - Baculoviridae. In: Virus Taxonomy. Elsevier. San Diego, CA, USA. pp. 163–173. Doi: 10.1016/B978-0-12-384684-6.00013-6. DOI: https://doi.org/10.1016/B978-0-12-384684-6.00013-6
Lucarotti, C. J., B. H. Whittome-Waygood and D. B. Levin. 2011. Histology of the Larval Neodiprion abietis (Hymenoptera: Diprionidae) Digestive Tract. Psyche: A Journal of Entomology e910286. Doi: 10.1155/2011/910286. DOI: https://doi.org/10.1155/2011/910286
Lucarotti, C. J., B. H. Whittome-Waygood, R. Lapointe, B. Morin and D. B. Levin. 2012. Pathology of a Gammabaculovirus in its natural balsam fir sawfly (Neodiprion abietis) host. Psyche: A Journal of Entomology e646524. Doi: 10.1155/2012/646524. DOI: https://doi.org/10.1155/2012/646524
Moreau, G., C. J., Lucarotti, E. G., Kettela, G. S., Thurston, S., Holmes, C., Weaver, D. B. Levin and B. Morin. 2005. Aerial application of nucleopolyhedrovirus induces decline in increasing and peaking populations of Neodiprion abietis. Biological Control 33: 65–73. Doi: 10.1016/j.biocontrol.2005.01.008. DOI: https://doi.org/10.1016/j.biocontrol.2005.01.008
Mosqueda, J., R. Cossío-Bayugar, E. Rodríguez, A. Falcón, A.Ramos, J. V., Figueroa, and A. Alvarez. 2008. Primary midgut, salivary gland, and ovary cultures from Boophilus microplus. Annals of the New York Academy of Sciences 1149: 49–52. Doi: 10.1196/annals.1428.050. DOI: https://doi.org/10.1196/annals.1428.050
Rohrmann, G. F. 2019. Baculovirus Molecular Biology. 4th Edition. National Center for Biotechnology Information. Bethesda, MD, USA. https://www.ncbi.nlm.nih.gov/books/NBK543458/ (6 junio de 2021).
Summers, M. D. and G. E. Smith. 1987. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station, College Station. Texas, USA. Bulletin 1555: 1-60. https://oaktrust.library.tamu.edu/handle/1969.1/145892 (6 de junio de 2021).
Wang, M. and Z. Hu. 2019. Cross-talking between baculoviruses and host insects towards a successful infection. Philosophical Transactions of the Royal Society B: Biological Sciences 374: 20180324. Doi: 10.1098/rstb.2018.0324.
Downloads
Published
Versions
- 2022-04-05 (2)
- 2022-01-10 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Revista Mexicana de Ciencias Forestales
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish in Revista Mexicana de Ciencias Forestales accept the following conditions:
In accordance with copyright laws, Revista Mexicana de Ciencias Forestales recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access.
All the texts published by Revista Mexicana de Ciencias Forestales –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned
The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Forestales (for example, include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Forestales.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: ciencia.forestal2@inifap.gob.mx
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.
